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mouse multiple tissue northern blot  (TaKaRa)


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    Structured Review

    TaKaRa mouse multiple tissue northern blot
    Mouse Multiple Tissue Northern Blot, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+multiple+tissue+northern+blots/Mouse+Eye+Total+RNA/pm39808388-14-16-23
    Average 95 stars, based on 47 article reviews
    mouse multiple tissue northern blot - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Hybridization:

    Article Title: A possible role of mouse Fused (STK36) in Hedgehog signaling and Gli transcription factor regulation
    Article Snippet: These fragments were amplified by PCR and radioactively labeled with 32 P-ATP using the High Prime DNA labeling kit (Boehringer Mannheim, Manheim, Germany) according to the manufacturer’s instructions. .. The hybridization was performed in ExpressHyb (Clontech) at 68°C for high specificity, to commercially available mouse Multiple Tissue Northern blots (Clontech), according to the recommendations by the manufacturer. ..

    Article Title: ZEC, a zinc finger protein with novel binding specificity and transcription regulatory activity.
    Article Snippet: A novel 114-kDa zinc finger protein, ZEC, has been found by cDNA cloning and characterized.. ZEC was strongly expressed in the testis, liver and kidney, and also in embryonic stem cells.. Epitope-tagged experiments indicated nuclear localization of ZEC.

    Northern Blot:

    Article Title: A possible role of mouse Fused (STK36) in Hedgehog signaling and Gli transcription factor regulation
    Article Snippet: These fragments were amplified by PCR and radioactively labeled with 32 P-ATP using the High Prime DNA labeling kit (Boehringer Mannheim, Manheim, Germany) according to the manufacturer’s instructions. .. The hybridization was performed in ExpressHyb (Clontech) at 68°C for high specificity, to commercially available mouse Multiple Tissue Northern blots (Clontech), according to the recommendations by the manufacturer. ..

    Article Title: ZEC, a zinc finger protein with novel binding specificity and transcription regulatory activity.
    Article Snippet: A novel 114-kDa zinc finger protein, ZEC, has been found by cDNA cloning and characterized.. ZEC was strongly expressed in the testis, liver and kidney, and also in embryonic stem cells.. Epitope-tagged experiments indicated nuclear localization of ZEC.

    Article Title: Fhos2, a novel formin-related actin-organizing protein, probably associates with the nestin intermediate filament.
    Article Snippet: Fhos1 is a mammalian formin-family protein, and functions as an organizer of the actin microfilament.. Here we have cloned human and mouse cDNAs for a novel Fhos homolog, designated Fhos2.. The messages for Fhos2 are expressed in the heart, kidney, and brain, where the Fhos1 mRNAs are not abundant.

    Article Title: Deficiency of hyccin, a newly identified membrane protein, causes hypomyelination and congenital cataract.
    Article Snippet: .. We probed human, human brain and mouse Multiple Tissue Northern Blots (Clontech) with P32 radiolabelled PCR products amplified from DRTCNNB1A. .. Hybridization was carried out overnight in ExpressHyb solution (Clontech) at 65°C.

    Article Title: Regulation of atrial natriuretic peptide secretion by a novel Ras-like protein
    Article Snippet: Isolation of RNA from brain, heart, skeletal muscle, and cells for Northern blot analysis and RT-PCR was performed using TRIzol according to the manufacturer's protocol (Invitrogen). .. Human and mouse multiple tissue Northern blots were purchased from CLONTECH Laboratories, Inc. DNA probes to detect human or mouse RRP17 transcripts were derived from heart cDNA using species-specific primers. .. For semi-quantitative PCR, cDNA was generated from 2 μg of total RNA with random-hexamer primers using the Superscript III kit (Invitrogen).

    Article Title: Tumor necrosis factor receptor-associated factors
    Article Snippet: .. To examine the tissue distribution of TRAF1 and TRAF2 mRNA, mouse multiple tissue Northern blots (Clontech) were hybridized with radiolabeled TRAF1 and TRAF2 probes according to the instructions of the manufacturer. ..

    Article Title: Gene Structure, Alternate Splicing, Tissue Distribution, Cellular Localization, and Developmental Expression Pattern of Mouse Deubiquitinating Enzyme Isoforms Usp2-45 and Usp2-69
    Article Snippet: Probes were labeled with [ 32 P]dATP by random primer extension using a Megaprime kit (Amersham). .. Mouse Multiple Tissue Northern blots were purchased from ClonTech and hybridized according to the manufacturer’s instructions. ..

    Polymerase Chain Reaction:

    Article Title: Deficiency of hyccin, a newly identified membrane protein, causes hypomyelination and congenital cataract.
    Article Snippet: .. We probed human, human brain and mouse Multiple Tissue Northern Blots (Clontech) with P32 radiolabelled PCR products amplified from DRTCNNB1A. .. Hybridization was carried out overnight in ExpressHyb solution (Clontech) at 65°C.

    Amplification:

    Article Title: Deficiency of hyccin, a newly identified membrane protein, causes hypomyelination and congenital cataract.
    Article Snippet: .. We probed human, human brain and mouse Multiple Tissue Northern Blots (Clontech) with P32 radiolabelled PCR products amplified from DRTCNNB1A. .. Hybridization was carried out overnight in ExpressHyb solution (Clontech) at 65°C.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Fhos2, a novel formin-related actin-organizing protein, probably associates with the nestin intermediate filament.
    Article Snippet: Fhos1 is a mammalian formin-family protein, and functions as an organizer of the actin microfilament.. Here we have cloned human and mouse cDNAs for a novel Fhos homolog, designated Fhos2.. The messages for Fhos2 are expressed in the heart, kidney, and brain, where the Fhos1 mRNAs are not abundant.

    Derivative Assay:

    Article Title: Regulation of atrial natriuretic peptide secretion by a novel Ras-like protein
    Article Snippet: Isolation of RNA from brain, heart, skeletal muscle, and cells for Northern blot analysis and RT-PCR was performed using TRIzol according to the manufacturer's protocol (Invitrogen). .. Human and mouse multiple tissue Northern blots were purchased from CLONTECH Laboratories, Inc. DNA probes to detect human or mouse RRP17 transcripts were derived from heart cDNA using species-specific primers. .. For semi-quantitative PCR, cDNA was generated from 2 μg of total RNA with random-hexamer primers using the Superscript III kit (Invitrogen).



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    Image Search Results


    (A, B) Northern blot analysis of multiple mouse tissue RNA shows messages for GSK3αand β are highest in testis. (C, D) Quantitative PCR showing increasing Gsk3α and Gsk3β mRNA levels in postnatal developing testis. The results are represented as fold change after normalizing the Gsk3α and Gsk3β mRNA levels with Gapdh mRNA. These data are representative of three independent experiments in triplicates, and error bars represent SE.

    Journal: Biology of Reproduction

    Article Title: Isoform-specific requirement for GSK3α in sperm for male fertility

    doi: 10.1093/biolre/ioy020

    Figure Lengend Snippet: (A, B) Northern blot analysis of multiple mouse tissue RNA shows messages for GSK3αand β are highest in testis. (C, D) Quantitative PCR showing increasing Gsk3α and Gsk3β mRNA levels in postnatal developing testis. The results are represented as fold change after normalizing the Gsk3α and Gsk3β mRNA levels with Gapdh mRNA. These data are representative of three independent experiments in triplicates, and error bars represent SE.

    Article Snippet: Mouse multiple tissue northern blot was obtained from Zyagen.

    Techniques: Northern Blot, Real-time Polymerase Chain Reaction